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Xianbing LIU, Can WANG
《环境科学与工程前沿(英文)》 2009年 第3卷 第1期 页码 12-19 doi: 10.1007/s11783-009-0011-x
关键词: CO2 embodiment international trade quantitative estimation analytical approach
null
《医学前沿(英文)》 2017年 第11卷 第1期 页码 97-109 doi: 10.1007/s11684-016-0496-1
As muscle activity during growth is considerably important for mandible quality and morphology, reducing dietary loading directly influences the development and metabolic activity of mandibular condylar cartilage (MCC). However, an overall investigation of changes in the protein composition of MCC has not been fully described in literature. To study the protein expression and putative signaling in vivo, we evaluated the structural changes of MCC and differentially expressed proteins induced by reducing functional loading in rat MCC at developmental stages. Isobaric tag for relative and absolute quantitation-based 2D nano-high performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight/ time-of-flight (MALDI-TOF/TOF) technologies were used. Global protein profiling, KEGG and PANTHER pathways, and functional categories were analyzed. Consequently, histological and tartrate-resistant acid phosphatase staining indicated the altered histological structure of condylar cartilage and increased bone remodeling activity in hard-diet group. A total of 805 differentially expressed proteins were then identified. GO analysis revealed a significant number of proteins involved in the metabolic process, cellular process, biological regulation, localization, developmental process, and response to stimulus. KEGG pathway analysis also suggested that these proteins participated in various signaling pathways, including calcium signaling pathway, gap junction, ErbB signaling pathway, and mitogen-activated protein kinase signaling pathway. Collagen types I and II were further validated by immunohistochemical staining and Western blot analysis. Taken together, the present study provides an insight into the molecular mechanism of regulating condylar growth and remodeling induced by reducing dietary loading at the protein level.
关键词: condylar cartilage mechanical loading proteomic analysis iTRAQ bioinformatics analysis
null
《医学前沿(英文)》 2016年 第10卷 第3期 页码 278-285 doi: 10.1007/s11684-016-0453-z
Colorectal cancer (CRC) is a leading cause of cancer-related deaths worldwide. We aimed to find novel molecules as potential biomarkers for the early diagnosis of CRC. A serum-free conditioned medium was successfully collected from three pairs of CRC tissue and adjacent normal tissue. iTRAQ-based quantitative proteomic analysis was applied to compare the differences in secretome between primary CRC mucosa and adjacent normal mucosa. A total of 145 kinds of proteins were identified. Of these proteins, 29 were significantly different between CRC and normal tissue. Tropomyosin 2 β (TPM2) exhibited the most significant differences; as such, this protein was selected for further validation. Quantitative real-time PCR indicated that the mRNA expression of TPM2 significantly decreased in the CRC tissue compared with the paired adjacent normal tissue. Immunohistochemical analysis also confirmed that TPM2 was barely detected at protein levels in the CRC tissue. In summary, this study revealed potential molecules for future biomarker applications and provided an efficient approach for the differential analysis of cancer-associated secretome. TPM2 may be valuable for the early diagnosis of CRC.
Quantitative analysis of microplastics in coastal tidal-flat reclamation in Dongtai, China
《环境科学与工程前沿(英文)》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1528-5
• Reclamation projects are important disturbances on microplastic risk in coasts.
关键词: Coastal reclamation Engineering geotextiles Soil microplastics Weathering simulation Marine emission
Jingjing WANG,Feng HUANG,Baoguo LI
《农业科学与工程前沿(英文)》 2015年 第2卷 第4期 页码 295-310 doi: 10.15302/J-FASE-2015074
关键词: AquaCrop summer maize soil water balance water productivity
《化学科学与工程前沿(英文)》 2022年 第16卷 第2期 页码 152-167 doi: 10.1007/s11705-021-2060-z
关键词: molecular property quantum mechanics quantitative structure-property relationship heat of reaction solid-liquid phase equilibrium
Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
《环境科学与工程前沿(英文)》 2011年 第5卷 第3期 页码 488-488 doi: 10.1007/s11783-011-0342-2
Estimation of photolysis half-lives of dyes in a continuous-flow system with the aid of quantitative
Davoud BEIKNEJAD,Mohammad Javad CHAICHI
《环境科学与工程前沿(英文)》 2014年 第8卷 第5期 页码 683-692 doi: 10.1007/s11783-014-0680-y
关键词: dye photolysis half-life quantitative structure-property relationship continuous-flow stepwise multiple linear regression partial least squares
Application of quantum chemical descriptors into quantitative structure-property relationship models
Yueping BAO, Qiuying HUANG, Wenlong WANG, Jiangjie XU, Fan JIANG, Chenghong FENG
《环境科学与工程前沿(英文)》 2011年 第5卷 第4期 页码 505-511 doi: 10.1007/s11783-011-0318-2
关键词: photolysis polychlorinated biphenyls (PCBs) quantitative structure-property relationships (QSPRs) quantum chemical descriptors
《农业科学与工程前沿(英文)》 2023年 第10卷 第2期 页码 306-332 doi: 10.15302/J-FASE-2023488
● Using visual analysis to predict the trend of natural product pest resistance.
关键词: anti-pest activity crop protection insect pest natural product visual analysis
Jinsong Tao, Zijian Li, Xinlai Peng, Gaoxiang Ying
《环境科学与工程前沿(英文)》 2017年 第11卷 第4期 doi: 10.1007/s11783-017-0952-4
关键词: Green stormwater infrastructure (GSI) Combined sewer overflows (CSOs) Urban flooding Low impact development (LID) Stormwater Management Model (SWMM)
Aerodynamic impact of train-induced wind on a moving motor-van
Jiajun HE; Huoyue XIANG; Yongle LI; Bin HAN
《结构与土木工程前沿(英文)》 2022年 第16卷 第7期 页码 909-927 doi: 10.1007/s11709-022-0833-1
关键词: rail-cum-road bridge aerodynamic impact train-induced wind CFD aerodynamic force quantitative analysis fitting
Design and use of group-specific primers and probes for real-time quantitative PCR
Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG
《环境科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 28-39 doi: 10.1007/s11783-011-0302-x
关键词: absolute quantification design guideline primer probe real-time quantitative polymerase chain reaction (qPCR)
Overview of the quality standard research of traditional Chinese medicine
Huimin Gao, Zhimin Wang, Yujuan Li, Zhongzhi Qian
《医学前沿(英文)》 2011年 第5卷 第2期 页码 195-202 doi: 10.1007/s11684-011-0134-x
关键词: traditional Chinese medicine quality standard quality control quantitative analysis of multi-components by single-marker
Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO
《环境科学与工程前沿(英文)》 2011年 第5卷 第2期 页码 175-185 doi: 10.1007/s11783-011-0320-8
关键词: quantitative polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) principal component analysis (PCA) canonical correspondence analysis (CCA)
标题 作者 时间 类型 操作
iTRAQ-based quantitative proteomic analysis on differentially expressed proteins of rat mandibular condylar
null
期刊论文
iTRAQ-based quantitative analysis of cancer-derived secretory proteome reveals TPM2 as a potential diagnostic
null
期刊论文
Quantitative analysis of yield and soil water balance for summer maize on the piedmont of the North China
Jingjing WANG,Feng HUANG,Baoguo LI
期刊论文
A computational toolbox for molecular property prediction based on quantum mechanics and quantitative
期刊论文
Erratum to: Using pyrosequencing and quantitative PCR to analyze microbial communities
Husen ZHANG
期刊论文
Estimation of photolysis half-lives of dyes in a continuous-flow system with the aid of quantitative
Davoud BEIKNEJAD,Mohammad Javad CHAICHI
期刊论文
Application of quantum chemical descriptors into quantitative structure-property relationship models
Yueping BAO, Qiuying HUANG, Wenlong WANG, Jiangjie XU, Fan JIANG, Chenghong FENG
期刊论文
QUANTITATIVE STUDY ON ANTI-PEST ACTIVITY OF NATURAL PRODUCTS BASED ON VISUALIZATION FRAMEWORK OF KNOWLEDGE
期刊论文
Quantitative analysis of impact of green stormwater infrastructures on combined sewer overflow control
Jinsong Tao, Zijian Li, Xinlai Peng, Gaoxiang Ying
期刊论文
Aerodynamic impact of train-induced wind on a moving motor-van
Jiajun HE; Huoyue XIANG; Yongle LI; Bin HAN
期刊论文
Design and use of group-specific primers and probes for real-time quantitative PCR
Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG
期刊论文
Overview of the quality standard research of traditional Chinese medicine
Huimin Gao, Zhimin Wang, Yujuan Li, Zhongzhi Qian
期刊论文